Journal: Translational Lung Cancer Research

Article Title: The downregulation of fibrinogen-like protein 1 inhibits the proliferation of lung adenocarcinoma via regulating MYC -target genes

doi: 10.21037/tlcr-22-151

Figure Lengend Snippet: The knockdown of FGL1 and RNA-seq results. (A) The knockdown of FGL1 confirmed by western blot. (B) The knockdown of FGL1 confirmed by immunofluorescent staining. (C) Volcano map of differential gene changes in PC9 cells after FGL1 knockdown. (D) Volcano map of differential gene changes in Jurkat T cells after FGL1 knockdown. (E) The enrichment of differential genes in PC9 cells, analyzed by GSEA. **, P<0.01; ***, P<0.001. NC, negative control; KD, knockdown; LUAD, lung adenocarcinoma; FGL1, fibrinogen-like protein 1.

Article Snippet: PC9 lung cancer cells and HCC827 lung cancer cells (human; iCell Bioscience Inc., Shanghai China) were cultured in RPMI 1640 (Gibco, Shanghai, China) with 10% fetal calf serum at 37 °C in 5% CO 2 .

Techniques: Knockdown, RNA Sequencing, Western Blot, Staining, Negative Control

Journal: Translational Lung Cancer Research

Article Title: The downregulation of fibrinogen-like protein 1 inhibits the proliferation of lung adenocarcinoma via regulating MYC -target genes

doi: 10.21037/tlcr-22-151

Figure Lengend Snippet: The experiments related to cell proliferation. (A,B) Effects of FGL1 knockdown on the cycle of PC9 cells and Jurkat T cells. (C) Real-time cell analyzer presents the real-time condition of cell proliferation. (D) Colony formation confirms the cell proliferation of PC9 cells and HCC827 cells, the magnifications in the figure are 40× and 200×, respectively, both of them were stained with crystal violet. **, P<0.01; ***, P<0.001; ****, P<0.0001. NC, negative control; KD, knockdown; LUAD, lung adenocarcinoma; FGL1, fibrinogen-like protein 1.

Article Snippet: PC9 lung cancer cells and HCC827 lung cancer cells (human; iCell Bioscience Inc., Shanghai China) were cultured in RPMI 1640 (Gibco, Shanghai, China) with 10% fetal calf serum at 37 °C in 5% CO 2 .

Techniques: Knockdown, Staining, Negative Control

Journal: Technology in Cancer Research & Treatment

Article Title: KRT6A Promotes EMT and Cancer Stem Cell Transformation in Lung Adenocarcinoma

doi: 10.1177/1533033820921248

Figure Lengend Snippet: Keratin 6A knockdown undermine the proliferation and migration ability of lung cancer cells. A and B, KRT6A was knocked down by siRNA, siRNA significantly knocked down KRT6A mRNA expression and protein level in A549 and PC-9 cell lines (the Western blots were quantified by Image J). C and D, CCK8 assay shown si-KRT6A could significantly undermine the proliferation of A549 and PC-9 cells. E, Would healing assay shown that KRT6A knockdown significantly undermined the migration ability in lung cancer cells. F, By measuring the supernatant LDH level, the cell death rate was determined, no significant difference was found between si-KRT6A and NC groups, which indicate KRT6A knockdown did not influence the cell death rate between NC and KRT6A knockdown group. CCK8 indicates Cell Counting Kit-8; KRT6A, keratin 6A; mRNA, messenger RNA; NC, negative control; siRNA, small interfering RNA.

Article Snippet: The lung cancer cell line, A549 was obtained from the ATCC (American Type Culture Collection) and PC-9 was obtained from NanJing KeyGen Biotech Co, Ltd. A549 and PC-9 cells were cultured in Dulbecco’s Modified Eagle’s Medium (Thermo Fisher Scientific, Inc, Waltham, MA; cat. no. 12491-015) supplemented with 10% fetal bovine serum (FBS; cat. no. 10500064; Gibco; Thermo Fisher Scientific, Inc) and penicillin/streptomycin cultured in a humidified incubator at 37°C and 5% CO 2 .

Techniques: Knockdown, Migration, Expressing, Western Blot, CCK-8 Assay, Cell Counting, Negative Control, Small Interfering RNA

Journal: Technology in Cancer Research & Treatment

Article Title: KRT6A Promotes EMT and Cancer Stem Cell Transformation in Lung Adenocarcinoma

doi: 10.1177/1533033820921248

Figure Lengend Snippet: Keratin 6A is involved in CSCs transformation. A and B, CXCR4 high /CD133 high cells were identified as CSCs, compared with si-KRT6A groups, NC groups have significantly expanded CSCs population (Q2). C and D, The colony formation assay shown that compared with si-KRT6A A549 and PC-9 cells the normal control cancer cells (NC groups) have greater biological advantage in forming colonies. CSC indicates cancer stem cell; KRT6A, keratin 6A; NC, negative control.

Article Snippet: The lung cancer cell line, A549 was obtained from the ATCC (American Type Culture Collection) and PC-9 was obtained from NanJing KeyGen Biotech Co, Ltd. A549 and PC-9 cells were cultured in Dulbecco’s Modified Eagle’s Medium (Thermo Fisher Scientific, Inc, Waltham, MA; cat. no. 12491-015) supplemented with 10% fetal bovine serum (FBS; cat. no. 10500064; Gibco; Thermo Fisher Scientific, Inc) and penicillin/streptomycin cultured in a humidified incubator at 37°C and 5% CO 2 .

Techniques: Transformation Assay, Colony Assay, Control, Negative Control

Journal: Scientific Data

Article Title: A single cell RNAseq benchmark experiment embedding “controlled” cancer heterogeneity

doi: 10.1038/s41597-024-03002-y

Figure Lengend Snippet: Cellranger statistics.

Article Snippet: PC9 (CSC-C4619J) human lung cancer cell line was purchased from Creative Bioarray; DV90 (ACC 307) and HCC78 (ACC 563) human lung cancer cell lines were provided from DSMZ Leibniz Institute.

Techniques:

Journal: Scientific Data

Article Title: A single cell RNAseq benchmark experiment embedding “controlled” cancer heterogeneity

doi: 10.1038/s41597-024-03002-y

Figure Lengend Snippet: Quality control of the cells. Low quality cells are characterised by having a low number of genes depicted as present, associated with low ribosomal content and high mitochondrial content. ( A ) A549, ( B ) CCL-185-IG, ( C ) CRL5868, ( D ) DV90, ( E ) HCC78, ( F ) HTB178, ( G ) PC9.

Article Snippet: PC9 (CSC-C4619J) human lung cancer cell line was purchased from Creative Bioarray; DV90 (ACC 307) and HCC78 (ACC 563) human lung cancer cell lines were provided from DSMZ Leibniz Institute.

Techniques: Control